News The localization of endophytes: new experiment for UNIMI

The localization of endophytes: new experiment for UNIMI

At the University of Milan, prelimary experiments have started to investigate the localization within Arabidopsis thaliana tissues of some endophytes isolated from Sicilian grapevine varieties. To do so, the University of Padua provided one of the isolated endophyte transformed with the green fluorescent protein (GFP) in order to visualize it with imaging techniques. Mainly two imaging approaches have been performed: mesoscopy (Fig. A) and confocal laser scanning microscopy – CLSM (Fig. B).

Arabidopsis thaliana seedlings, expressing the genetically encoded Ca2+ indicator (R-GECO1, red), were grown in plates on agar medium. Droplets of a suspension cultures of a grapevine isolated endophyte expressing GFP were applied to the root tip of 7-day-old Arabidopsis seedlings (green) (Fig. A). After 48 hours from inoculation, the shoot was anlyzed by means of contocal microscopy which revelead the presence of green fluorescence puncta corresponding to fluorescent endophytes (Fig. B and C).

Red fluorescence represents the cytosolic localized R-GECO1 fluorescence,  of leaf epidermal cells. Green fluorescent puncta are GFP expressing endophytes.

Related Post